HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. For HTRF readings, a 620/40 nm filter (PerkinElmer 2100-5760) and a 665/7. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. 5 µL Prostaglandin E2 . Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. and Canaa -8-963-4567. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. For a list of HTRF-compatible readers and set-up recommendations, please visit . 5% and an increased Z’ value of 0. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. Plate 25 μL of cells in. MATERIALS PROVIDED: KIT . The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For more information about HTRF. Read & analyse. ** The Detection Buffer is used to prepare working solutions of acceptor and donor reagents. Optimized instrument settings for the HTRF camps HiRange assay. Read & analyse. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. Read & analyse. • HTRF®-Certified Reader. culture plate. BMG LABTECH microplate reader; Early passage Human Osteoblast cells (HOb 406-05a), supplied by the European Collection of Cell Cultures ECACC were seeded onto substrates A and B at a density of 10 4 per sample. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. Remove the plate seal and read on an HTRF compatible reader. Remove the plate seal and read on an HTRF compatible reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For more information. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. → Incubate 2h (if the detection is not sensitive enough, it is better to incubate 20h). Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It can also be further streamlined to a one-. Revvity. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. FOR HTRF CERTIFIED. Discover the features and benefits of the VICTOR Nivo multimode plate reader, the smallest and most versatile instrument for life science applications. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. Rating: 5. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. 4. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. 27 / Pack of 100; Qty. This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. Customer Transformation Hub. HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. best fluorescence polarization plate reader on the market. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. Read & analyse. Its. Plate 25 μL of cells in. Neocarzinostatin dose-response on Jurkat cells. CLARIOstar is equipped with a specific optical device which enables the measurement of. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Customer Transformation Hub. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. FOR HTRF CERTIFIED READER . Experimental details• HTRF®-Certified Reader **. Assay detection requires a microplate reader with. 5 hours before a further 3 washing steps. The FLUOstar Omega is the ideal plate reader for life science applications. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. The kit includes everything you need: reagents, buffers and a microplate. For fluorescent assays, black plates absorb light and reduce background and crosstalk. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 96-well culture plate 384SV detection plate- Add . Discover our high performance reader. These plates can be read on all HTRF certified readers, thus making their implementation in. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla (Figure 1). EX: HTRF Emission range. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total Cereblon HTRF detection reagents. 3nm, important when reading at 280 nm for DNA analysis, for more exact results. HTRF 96 TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: 3h * * Depending on cell lines used, volume of lysis should be optimized. * For HTRF microplate recommendations, please visit . The Spark multimode reader from Tecan performs accurate and sensitive measurements of fluorescent cell-based assays. For more information about HTRF. The Bradford assay was successfully performed on the BMG LABTECH microplate reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Lysates are then transferred to the assay plate for the detection of Total-eNOS by HTRF. In this case the Z´(pronounced Z prime) value comes to mind. FOR HTRF CERTIFIED READER For more. 5 nm filter (PerkinElmer 2100-5110) were used. 6% for 1,408 wells and a Z’ value of 0. This protocol, HTS designed, allows miniaturization while. This protocol gives the cells viability and confluence to be monitored. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The plate was covered both incubated for one hour at room temperature. Learn about the principles, applications, and benefits of HTRF in this Agilent application note. PerkinElmer. Read & analyse. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. → Cover the plate with a plate sealer. No washing steps are required. FOR HTRF CERTIFIED READER. + 4 µL HTRF ® pre-mixed antibo dies 2. Up to 8 decades dynamic range in one measurement with EDR technology. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:FOR HTRF CERTIFIED READER. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. It features a modular design that allows users to add or change detection cartridges according to their needs. Lysates are then transferred to the assay plate for the detection of total-CDK4 by HTRF. For instance, when assay volumes are. FOR HTRF CERTIFIED READER For more information about HTRF. Plate 25 μL of cells in. • HTRF®-Certified Reader **. Remove the plate seal and read on an HTRF compatible reader. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". Assay setup for a 384-well low-volume plate. The plate was covered and incubated for two hours at room temperature. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Synergy H1 offers researchers a choice of monochromators or filters or both detection technologies in one compact unit. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. Plate 25 μL of cells in. HTRF-specific. Preconfigured HTRF. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. The plate map included a low and a high level calibrator, conjugate blanks, and a specific control to assess the counting rate at 620 nm (i. It can also be further streamlined toFOR HTRF CERTIFIED READER. There are multiple factors to consider including: detection type, additional. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. White 384-well low volume plate Make sure to use the set-up for Eu3+ Cryptate. If not, our scientific team will help you choose the instrument that best suits your needs and budget. The ratio for each well must be calculated individually. FOR HTRF CERTIFIED READER. It can also be further streamlined to a one-. SERIAL DILUTIONS . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. These plates can be read on all HTRF certified readers, thus making their implementation in. The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 9. ® Detection of Progesterone Receptor with HTRF reagents is performed in a single plate used for plating, stimulation and detection. 45mm 5000µs Delay time Reading time Operation mode 100 µs 400 µs by plate 100 µs 400 µs by plate • This reader only allows high performance HTRF measurement when assays are run in WHITE plates. The optical systems use two scanning All the benefits of HTRF with ELISA plate ease of use. For more information about HTRF. • ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. Set up your reader for Eu3+ Cryptate and read the fluorescence emission at two different wavelengths (665nm and 620nm) on a compatible HTRF® reader**. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detectionThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). Plate 25 μL of cells in. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Measure elevated and normal expression samples without multiple dilutions. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. A gradient of signal is apparent prior to plate reader optimization (a) causing a %CV of 9. Reagents were dispensed following Cisbio’s ligand binding protocol (Figure 3). Live Viewer for cell-based assays. Homogeneous Time-Resolved Fluorescence (HTRF) . The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. FOR HTRF CERTIFIED READER. These plates can be read on all HTRF certified readers, thus making their implementation in. The ratio of the two fluorescence TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. FOR HTRF CERTIFIED READER. Assay plate setup. Make sure the setup for Eu 3+ Cryptate is used. FOR HTRF CERTIFIED READER For. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. The Negative control is used to check the non-specific signal. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. (In the following text, the term YTHDC1 is used frequently as an abbreviation for the m 6 A-recognition domain of YTHDC1). HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Lysates are then transferred to the assay plate for the detection of total-CREB by HTRF. 15 / Each of 1; Qty. The PHERAstar ® FSX and CLARIOstar ® microplate readers offer the best possible performance for AlphaScreen ®, AlphaLISA ®, and AlphaPlex™ assays. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. Incubate overnight at RT. FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Remove the plate seal and read on an HTRF compatible reader. For more information about HTRF ®Seal the plate and incubate for Overnight. FOR HTRF CERTIFIED READER. Make sure the setup for Eu Cryptate is used. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. For more information about HTRF. * For HTRF microplate recommendations, please visit Table 1 Instrument settings used for HTRF with terbium donor and red acceptor. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. FOR HTRF CERTIFIED READER. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications and can be further upgraded to a multi-mode reader. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. FOR HTRF CERTIFIED READER For more. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Step 5 Remove the plate sealer and read on an HTRF® compatible reader The blank control is used to check the Cryptate signal at 620 nm. 96-well culture plateFOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. Incubate 2h at RT Transfer 16 µL supernatant. The EnVision XCite Plate Reader provides exceptional sensitivity and speed with minimal downtime - backed by our global network of application scientists and engineers. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Transcreener® ADP2 FP assay. -CERTIFIED READER IS NEEDED. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. FOR HTRF CERTIFIED READER. Discover our portfolio of multimode plate readers and radiometric detection solutions, complemented by assays, reagents and consumables which together enable you to increase your lab’s. Each well requires 10 μL of 4X lysis buffer /well. Lysis buffer 1X for assay protocol on tissues, two-plate assay protocol on adherent cells, and standard dilution. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The measurement of HTRF assays is predominantly performed on microplate readers. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. • HTRF®-Certified Reader **. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. acceptor reagent. Combined with our Kaleido™ workflow software, it. 96-well culture plate+ 4 µL HTRF pre-mixed antibodies. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. It continues to deliver optimal results. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Make sure the setup for Eu3+ Cryptate is used. Choosing the right microplate reader ensures you’ll get an optimal readout. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. The emission of light by the acceptor will be proportional to the level of interaction. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. Designed to the highest standards to support your drug discovery research, Corning ® and Falcon ® microplates are ideal for drug discovery applications, including assay prep, assay development, and HTS and automation. FOR HTRF CERTIFIED READER. • HTRF®-Certified Reader. (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). best fluorescence polarization plate reader on the market. HTRF is compatible with a large range of available microplate readers. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. HTRF plate reader. Phospho-Rb (Ser780) two-plate assay protocol. For more information about HTRF ®• HTRF®-Certified Reader. The plate is set up for reading and images are acquired according to specified parameters. Highly compatible plates for HTRF. Seal the plate and. Nephelometers are dedicated instruments that can only detect scattering and are only available as standalone plate readers. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. These plates can be read on all HTRF certified readers, thus making their implementation in. FOR ORE INFORMATION Europe and other ountries 33(0)466 96 0 U. Lysates are then transferred to the assay plate for the detection of Total Cofilin by HTRF® reagents. 1. Microplate readerFluorescence microplate readers usually contain a light source, an optical system for the selection of excitation light (i. For instance, when assay volumes are. For more information about set-up and compatible HTRF ® readers, please visit our website at: ® pre-mixed antibodies TWO- PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells . VICTOR® Nivo™ multimode plate reader 2 | For research use only. 55 µL anti-cAMP-Cryptate. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Its. Cover the plate with a plate sealer. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). It features a modular design that allows users to add or change detection cartridges according to their needs. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Seal the plate and incubate 3 hours at RT . 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. On BMG LABTECH´s NEPHELOstar Plus, a laser diode (at 635 nm) with a highly collimated beam is used as high-intensity light source. Read & analyse. PROTOCOL AT A GLANCE. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. This protocol gives the cells viability and confluence to be monitored. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Make sure the setup for Tb Cryptate is used. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. Read & analyse. Typical applications The HTRF cartridge provides optimal performance for key applications. 1) . Place the imaging filter cube on top of the LED cube you installed, and use the hex wrench to screw it to the LED cube. For multiplexed luminescent and fluorescent assays, using a white plate will support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for the fluorescent portion of the assay. Incubate 4h at RT. HTRF Microplate Readers. For more information about HTRF. Other technologies such as imaging. Using a black. The laser beam passes through the sample well. Cells in each image are identified by SoftMax Pro Software and cell-by-cell HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. At Cisbio we provide microplates that help you discover better. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. Its. FOR HTRF CERTIFIED READER. The sample is then added and incubated 1. VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. It fits any benchtop – and budget Building on our pioneering family of benchtop multimode plate readers, the VICTOR® Nivo™ multimode reader breaks new ground, packing all popular detection modes into the industry’s smallest benchtop. They can be used in a no-transfer protocol with suspension cells or with. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Revvity. February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. The FLUOstar ® Omega is a multi-mode microplate reader with three detection modes. FOR HTRF CERTIFIED READER For. On the SpectraMax i3x reader, install the HTRF cartridge, and wavelengths will be selected automatically when HTRF is selected as the Optical Configuration. For more. Plus . The tried, tested, and proven EnVision plate reader has an array of exciting features that can enhance your applications. The kit includes everything you need: reagents, buffers and a microplate. For a list of HTRF-compatible readers and set -up recommendations, please visit. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. For more information about HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It can also be further streamlined toLysates are then transferred ®to the assay plate for the detection of Total TYK2 by HTRF reagents. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. If not, our scientific team will help you choose the instrument that best suits your needs and budget. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. When equipped with a quad monochromator, the EnSight plate reader can improve performance for absorbance and fluorescence detection. Plate 25 μL of cells in. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Plate cells Stimulate cells 3. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. 3-in-1 absorbance using a variety of sample formats, including microplates. The assay was prepared in both white and black plates and incubated >18 hours. The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Fluorescence intensity – Fluorescence intensity is one of the most popular detection modes, and there are innumerable fluorophores available for applications such as DNA or protein quantitation, reporter-geneCertified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Plus . The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Envision Nexus. -Certified Reader. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates to a low volume detection plate (either HTRF 384-lv or 96-lv plate) before the addition of HTRF Phospho-Rb (Ser780) detection reagents. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Alpha Technology: The VICTOR Nivo system. The controls are equivalent to the upper and lower limit of. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Plate 25 μL of cells in. It comes equipped with our patented LVF Monochromators™, filters, and spectrometer. 2. HTRF is a FRET-based technology that emerged from the Jean-Marie Lehn ’s Nobel prize-winning work on rare earth complexes. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development. Simply place the module into one of the five module bays and read the assay. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. FOR HTRF CERTIFIED READER For more information about HTRF. Synergy NEO2 readers must be appropriately configured for HTRF® readout by setting up the measurement conditions in the Gen5™ Reader Control and Data Analysis Software. 8 µL cells. This protocol, HTS designed, allows miniaturization while. Eu cryptate emission level). 14 seconds for a 384-well plate. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate AntibodyCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Dedicated UV-laser excitation. For more information about HTRF. This protocol, HTS designed, allows miniaturization while. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the. For a list of HTRF-compatible readers and set-up recommendations, please visit . It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. Assay plates were incubated at RT and read at different time points thereafter on an HTRF ® certified. -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. For more information about HTRF ®Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. BMG LABTECH’s UV-laser not only allows for faster read times in europium TRF assays, but it also greatly enhances performance in terbium-based TRF assays. PerkinElmer.